Optimization of extraction method of total flavones in Lonicera japonica by orthogonal design

VernacularTitle:正交试验法优选金银花总黄酮的提取工艺
Author: Siyong YE ; Zhi CHEN
Keywords: Flos lonicerae japonicae; Flavones; Orthogonal test; Solvent extraction
From:International Journal of Traditional Chinese Medicine 2017;39(9):820-823
CountryChina
Language:Chinese
Abstract: Objective To optimize and establish the extraction of total flavones in Lonicera japonica. Methods The ultraviolet spectrophotometry was used to determinate the total flavonoids. The effect of extraction time, the amount of solvent and extraction times,pH were tested by the orthogonal design.ResultsThe total flavonoids showed a linear relationship,the optimal extraction condition of total flavones in Lonicera japonica was with 16 times volume of sample weight water for three times, each time was 2 h.Conclusions The method is simple and applicable to the extraction of total flavones in Lonicera japonica.