Expression of Phosphatidylinositol 3-Kinase, Protein Kinase B and Mammalian Target of Rapamycin in Substantia Nigra in Rats with Parkinson's Disease
10.3969/j.issn.1006-9771.2017.09.011
- VernacularTitle:磷脂酰肌醇3激酶、蛋白激酶B、哺乳动物雷帕霉素靶蛋白在帕金森病模型大鼠黑质区的表达
- Author:
Xinyue HU
;
Bin LIU
;
Jiali XU
;
Jinxia ZHANG
- Publication Type:Journal Article
- Keywords:
Parkinson's disease;
phosphatidylinositol 3-kinase;
protein kinase B;
mammalian target of rapamycin;
substantia nigra;
rats
- From:
Chinese Journal of Rehabilitation Theory and Practice
2017;23(9):1043-1050
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the expression of phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway in substantia nigra in rats with Parkinson's disease (PD). Methods A total of 96 Sprague-Dawley rats were randomly divided into sham group, model group, PI3K inhibitor LY294002 group and mTOR inhibitor rapamycin group. Each group was divided into 4 days and 8 days subgroups after the model. PD model was established by injecting rotenone subcutaneously. The expres-sion of PI3K, p-Akt and p-mTOR in substantia nigra was detected with immunohistochemistry and Western blotting. Results Compared with the sham group, the expression of PI3K, p-Akt and p-mTOR increased in the model group (P<0.05), and was more in 8 days subgroup than in 4 days subgroup (P<0.05). Compared with the model group, the expression of p-Akt and p-mTOR reduced in the LY294002 group (P<0.05), while the expression of PI3K varied little (P>0.05);the expression of p-mTOR decreased in the rapamycin group (P<0.05), while the expression of PI3K and p-Akt varied little (P>0.05). Conclusion PI3K/Akt/mTOR signaling pathway is over activated in substantia nig-ra in rats with Parkinson's disease, which may play an important role in occurrence and development of the disease.
