Roles of PIF1 helicase in cell cycle arrest induced by ionizing radiation
10.7644/j.issn.1674-9960.2017.03.003
- VernacularTitle:PIF1解旋酶在电离辐射诱发的细胞周期阻滞中的作用
- Author:
Zhongqiu LI
;
Chao LI
;
Degen ZHAO
;
Yang YANG
;
Xueping LI
;
Yan ZENG
;
Xiujie PAN
;
Zhihua YANG
;
Pingkun ZHOU
;
Maoxiang ZHU
;
Yongqing GU
- Keywords:
PIF1 helicase;
radiation;
ionizing;
radiation sensitivity;
cell cycle arrest;
DNA damage
- From:
Military Medical Sciences
2017;41(3):169-173,177
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of PIF1 knockdown on cell growth and cell cycle arrest induced by ionizing radiation.Methods HeLa cell lines that consistently down-regulated PIF1 were prepared by the lentivirus granules interfering technology and confirmed by real-time PCR and Western blotting.The effect of down-regulation of PIF1 on cell growth and cell cycle arrest induced by ionizing radiation was evaluated by cell counting and flow cytometry.Results HeLa cell lines consistently down-regulating PIF1 were established.The growth of HeLa that down-regulated PIF1 was inhibited greatly after 4 Gy of γ-ray irradiation.There was little cell proliferation until the 5th day post 4 Gy γ-ray.Moreover, the S phase block and G2/M phase block of PIF1 knock-downed cell lines were significantly delayed after 8 Gy γ-ray irradiation.Conclusion Knockdown of PIF1 can significantly enhance the radiation sensitivity and delayes the S phase block and G 2 /M phase block induced by ionizing radiation.
