Contents Determination of Chlorogenic Acid and Vitexin in Lophatherum gracile by HPLC
10.6039/j.issn.1001-0408.2016.06.36
- VernacularTitle:HPLC法同时测定淡竹叶中绿原酸和牡荆素的含量
- Author:
Haiyan SHI
;
Nan XU
;
Yutuan WANG
;
Lequn SU
;
Hongjian LI
- Publication Type:Journal Article
- Keywords:
Lophatherum gracile;
Chlorogenic acid;
Vitexin;
Content determination
- From:
China Pharmacy
2016;(6):833-834,835
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the simultaneous determination of chlorogenic acid and vitexin in Lophather-um gracile. METHODS:HPLC was performed on the column was Waters Atlantis C18 with mobile phases of acetonitrile- water (gradient elution)at a flow rate of 1.0 ml/min,detection wavelength was 280 nm,column temperature was 35 ℃,and the injec-tion volume was 10 μl. RESULTS:The linear range was 0.041 0-1.228 8 μg for chlorogenic acid(r=0.999 8)and 0.264 0-7.920 0μg for vitexin(r=0.999 9);RSDs of precision, stability and reproducibility tests were lower than 2%;recoveries were 97.6%-102.3%(RSD=1.85%,n=9) and 97.1%-101.3%(RSD=1.19%,n=9),respectively. CONCLUSIONS:The method is simple,stable and reproducible,and can be used for the simultaneous determination of chlorogenic acid and vitexin in L. gracile.
