Qualitative Identification and Content Determination of Berberine Hydrochloride in Huangjin Paste
10.6039/j.issn.1001-0408.2015.30.30
- VernacularTitle:黄金膏中盐酸小檗碱的定性鉴别与含量测定Δ
- Author:
Liang CHEN
;
Wei LIU
;
Chaolun GE
;
Honglin TIAN
- Publication Type:Journal Article
- Keywords:
Huangjin paste;
Berberine hydrochloride;
TLC;
HPLC
- From:
China Pharmacy
2015;(30):4262-4263,4264
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the qualitative identification and content determination of berberine hydro-chloride in Huangjin paste. METHODS:TLC was adopted for the qualitative identification of berberine hydrochloride and HPLC was conducted for the content determination of berberine hydrochloride in preparation. The column was Symmetry Shield Rp-18 with mobile phase of acetonitrile-0.1% phosphoric acid(50∶50,V/V)at a flow rate of 1.0 ml/min,detection wavelength was 265 nm,column temperature was 30 ℃ and injection volume was 10 μl. RESULTS:TLC spots of berberine hydrochloride in prepara-tion were clear and well-separated. The linear range of berberine hydrochloride was 2.5-20.0 μg/ml(r=0.999 0);RSDs of preci-sion,stability and reproducibility tests were lower than 3%;recovery was 97.7%-102.1%(RSD=1.68%,n=6). CONCLUSI-ONS:The method is simple,accurate and reproducible,and can be used for the qualitative identification and content determination of berberine hydrochloride in Huangjin paste.
