Effect and mechanism of total flavones of oldenlendia diffusa willd on epithelial-mesenchymal transition of cell line MHCC97-H induced by TGF-β1
10.7652/jdyxb201602027
- VernacularTitle:白花蛇舌草总黄酮对TGF-β1诱导的肝癌MHCC97-H细胞EMT的逆转作用及其机制
- Author:
Yanbing ZHANG
;
Jiao ZHU
;
Juxiang XIAO
;
Yahuan GUO
;
Zijun LIAO
;
Rui XU
- Publication Type:Journal Article
- Keywords:
MHCC97-H cell;
transforming growth factor-β1 (TGF-β1 );
epithelial-mesenchymal transition;
total flavones of oldenlandia diffusa willd;
invasion;
E-cadherin;
vimentin
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
2016;(2):279-282,306
- CountryChina
- Language:Chinese
-
Abstract:
ABSTRACT:Objective To investigate the effects of total flavones of oldenlandia diffusa (FOD)on epithelial-mesenchymal transition in hepatocellular cancer cell line MHCC97-H.Methods TGF-β1 induced EMT in routinely cultured liver cancer cell line MHCC97-H;then MHCC97-H cell was divided into 5 groups:normal control group, TGF-β1 group,TGF-β1 + FOD group,TGF-β1 + 5-FU group,and TGF-β1 + FOD + 5-FU group.After 48 h of treatment,the invasion ability of MHCC97-H cell was detected by Transwell;the proteins of E-cadherin and vimentin were determined by Western blot.Results Compared with the normal form of MHCC97-H cell line,the cell had obvious long fusiform after TGF-β1 induction,and the invasion ability enhanced (P = 0.02 ).But after treatment,the invasion ability of MHCC97-H cell decreased in FOD group and 5-FU group compared with that in TGF-β1 group (P = 0.03,P = 0.02 ),and decreased more significantly in FOD + 5-FU group (P = 0.01 ).The expression of E-cadherin at the protein level decreased significantly (P = 0.01 )in TGF-β1 group,which was abolished in FOD group (P =0.03 )and 5-FU group (P = 0.02 ).The expression of vimentin at the protein level increased significantly (P =0.01)in TGF-β1 group,which was abolished in FOD group (P =0.04)and 5-FU group (P =0.03)and more obviously in FOD+5-FU group (P =0.01).Conclusion FOD can reverse the invasion of MHCC97-H cells in EMT induced by TGF-β1 through decreasing the expression of E-cadherin protein and inhibiting the epithelial-mesenchymal transition of MHCC97-H cell.
