Optimization of processing parameters for extraction of chlorogenic acid in Fule Granula using Box-Behnken experimental design
10.3969/j.issn.1005-1678.2016.04.62
- VernacularTitle:Box－Behnken效应面法优化妇乐颗粒中绿原酸提取工艺
- Author:
Jiamiao LIN
;
Jianwei JIANG
- Publication Type:Journal Article
- Keywords:
Fule Granula;
lonicerae japonicae caulis;
chlorogenic acid;
extraction technology;
Box-Behnken experimental design
- From:
Chinese Journal of Biochemical Pharmaceutics
2016;36(4):196-198,202
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the processing parameters for the extraction of chlorogenic acid in Fule Granula by Box-Behnken experimental design.Methods The content of chlorogenic acid in Fule Granula was determination.The separation was performed on a Agilent ZORBAX Eclipse XDB C18 column(4.6 mm ×250 mm, 5 μm), and column temperature 30 ℃.Aetonitrile-0.2% H3PO4 was used as the mobile phase.The detection wavelength was at 327 nm.A three-factor and three-level Box-Behnken experimental design was employed to investigate effects of extraction time, solid-liquid ratio and extraction times on composite score of extracting amount of chlorogenic acid.Results Optimum process conditions were as follows:12 times the amount of water extracted three times, each time 60 min; chlorogenic acid extraction rate of 3.379%.Conclusion This optimized extraction technology has good predictability by Box-Behnken experimental design, and overall desirability, it provides a reference for application of Fule Granula.
