Effects of Pollen Typhae Total Flavone on Inflammatory Factors and Insulin Sensitivity in Type 2 Diabetic Rats
10.13359/j.cnki.gzxbtcm.2014.06.019
- VernacularTitle:蒲黄总黄酮对2型糖尿病大鼠炎症因子和胰岛素敏感性的影响
- Author:
Xiaotao FENG
;
Qun CHEN
;
Xiao LIANG
- Publication Type:Journal Article
- Keywords:
Type 2 diabetes/TCD therapy;
Insulin resistance;
Pollen Typhae total flavone/pharmacology;
Insulin tolerance;
Inflammatory factors;
Disease models,animal;
Rats
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2014;(6):936-939,943
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Pollen Typhae total flavone ( PTF) , an extract from Pollen Typhae which has the actions of activating blood and removing stasis, on inflammatory factors and insulin sensitivity in type 2 diabetic rats. Methods SD rats were used as the experimental animal. Type 2 diabetic rats induced by high fat diet plus low dose of streptozotocin were randomly divided into model group, PTF group (in the dosage of 200 mg·kg-1·d-1) , and rosiglitazone group (in the dosage of 4 mg·kg-1·d-1) . Additionally, normal control group was set up. After treatment for 4 weeks, plasma interleukin 6 ( IL-6) and tumor necrosis factor alpha ( TNF-α) levels were detected, the insulin tolerance test ( ITT) was performed, and the protein expression of suppressor of cytokine signaling-3 ( SOCS-3) in skeletal muscle was determined. Results After treatment for 4 weeks, the plasma levels of IL-6 and TNF-α, the homeostasis model of insulin resistance ( HOMA-IR) , and expression level of SOCS-3 in skeletal muscle in the model groups were significantly increased ( P﹤0.05) as compared with those in the normal control group, and insulin tolerance was also impaired in the model group ( P﹤0.05) . Compared with the model group, IL-6 level and HOMA-IR were markedly decreased in the PTF group ( P﹤0.05) , and the impaired insulin tolerance was obviously improved (P﹤0.05) . The level of SOCS-3 in the skeletal muscle of PTF group was also much lower than that of the model group and rosiglitazone group (P﹤0.05) . Conclusion PTF has effects on decreasing the levels of plasma IL-6 and SOCS-3 in the skeletal muscle and on improving insulin sensitivity in type 2 diabetic rats.
