Inhibition effect of flavonoids from Radix tetrastigmae on acute lung injury of aged mice through p38MAPK and NF-κB pathway
10.3969/j.issn.1001-1978.2015.12.019
- VernacularTitle:三叶青黄酮经 p38MAPK 和 NF-κB 途径抑制老年小鼠急性肺损伤
- Author:
Dandan LIU
;
Gang CAO
;
Qi ZHANG
;
Yilu YE
;
Likai HAN
;
Weihong GE
- Publication Type:Journal Article
- Keywords:
Radix tetrastigmae;
flavonoids;
p38MAPK;
nuclear factor-κB (NF-κB);
lipopolysac-charide (LPS);
acute lung injury (ALI);
aged mice
- From:
Chinese Pharmacological Bulletin
2015;(12):1725-1729,1730
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the protective effect of flavonoids from Radix tetrastigmae (RTFs)on lipopo-lysaccharide (LPS)induced acute lung injury (ALI) of aged mice and the mechanism.Methods Aged C57BL/6J mice were bronchially instillated LPS to in-duce ALI.RTFs were orally administered to treat ALI. After 3 days,bronchoalveolar lavage fluid (BALF) was collected to enumerate leukocytes with Wright-Gi-emsa staining,and to detect inflammatory cytokines with ELISA.ELISA and Western blot methods were al-so used to detect the expression of MAPKs and NF-κB in lung tissues.The activity of NF-κB in nucleic pro-tein extract was detected with TransAMkit.Results
ALI models were successfully induced through LPS in-stillation.RTFs significantly reduced leukocyte,espe-cially neutrophil infiltration in BALF,inhibited IL-1 β, IL-6,IL-1 2p40,TNF-αand sTNF-R1 secretion,and improved pathohistological change of lung tissues.Be-sides,RTFs significantly attenuated the phosphoryla-tion of p38MAPK,NF-κB and the activity of NF-κB. Conclusion RTFs inhibits LPS-induced ALI through p38MAPK and NF-κB pathway and exhibits significant anti-inflammation effect on aged mice.
