Neuroprotective of carnosine on oxygen-glucose deprivation/reperfusion induced injury in rat brain slices
- VernacularTitle:肌肽对大鼠脑片缺氧缺糖／再灌损伤的保护作用
- Author:
Chao FANG
;
Qing LI
;
Meili LU
;
Guoxing HUANG
;
Jing YANG
- Publication Type:Journal Article
- Keywords:
carnosine;
brain slices;
OGD/RP;
energy metabolism;
oxidative stress
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;(9):41-43,47
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate effect of carnosine on oxygen glucose deprivation/reperfusion ( OGD/RP) induced injury in rat brain slices. Methods Injury of brain slices was determined by TTC methods.The contents of ATP, ADP and AMP were determined by high performance liquid chromatography.Reactive Oxygen species ( ROS) were determined by fluorescence methods.Results Compared with control group, rat hippocampal slices were significantly damaged by OGD/RP, indicated by light color and decreased A490 nm value of TTC staining.Meanwhile the contents of ATP and ADP were significantly decreased, and the content of AMP and ROS were significantly increased, the difference between two group was significant ( P<0.01).Pre-incubation with Carnosine (1000, 200, 40 μg/mL) significantly inhibited the light color and decreased A490 nm value of TTC staining, increased the contents of ATP, ADP and AMP, and decreased the content of ROS, the difference between two group was significant ( P <0.01 ) . Conclusion Carnosine can protect rat hippocampal slices against injury induced by OGD/RP, which may relate to improve the energy metabolism and strengthen the ability of anti-oxidative stress.
