Neuroprotective effect of osthole on neuron synapses infected APP gene
10.3969/j.issn.1001-1978.2015.10.012
- VernacularTitle:蛇床子素对感染APP基因的神经元突触的保护作用
- Author:
Shaoheng LI
;
Yanan JIAO
;
Yingjia YAO
;
Liang KONG
;
Zhenyu TAO
;
Yuhui YAN
;
Jingxian YANG
- Publication Type:Journal Article
- Keywords:
osthole;
neurons;
synapses;
infection;
Alzheimer’ s disease;
CAMKK2/AMPK signal pathway
- From:
Chinese Pharmacological Bulletin
2015;(10):1383-1387,1388
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of osthole on neuron synapses infected APP gene and its underlying mechanism. Methods The neurons were divided into three groups:GFP, APP, APP+Ost groups. The neu-rons were infected APP gene with containing mutational site in vitro for mimicking the characterstics of Alzhei-mer’ s disease ( AD) . The cell viability was assessed by CCK-8 , the expression of synapsin-1 was deter-mined by immunofluorescence, and the concentration of PSD-95 and SYP were detected by ELISA. The ex-pressions of Aβ1-42 , CAMKK2 , phoshorylated AMPKα1 , AMPKα1 protein were determined by West-ern blot. Results Strong APP staining was visible in neurons infected with APP and abundant expression of Aβ1-42 , a neurotoxic oligomer. Compared with APP group, APP+Ost group significantly increased cell vi-ability, promoted the expression of synapsin-1, up-reg-ulated the concentration of PSD-95 and SYP, and de-creased the expressions of CAMKK2 and p-AMPKα1 . Conclusions Ost can protect the neuron synapses a-gainst infected with APP gene. Its neuroprotective effect may be related to inhibiting the CAMKK2/AMPK signal pathway.
