Effects of proliferation and apoptosis of phloretin on the hepatoma carcinoma cells HepG-2
- VernacularTitle:根皮素对肝癌细胞HepG－2增殖和凋亡的影响
- Author:
Hui WANG
;
Zheng LIU
;
Handong WU
- Publication Type:Journal Article
- Keywords:
phloretin;
human hepatoma cells;
apoptosis;
proliferation
- From:
Chinese Journal of Biochemical Pharmaceutics
2015;37(7):39-42,46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate proliferative and apoptotic effects of phloretin on hepatoma carcinoma cells, hepatoma carcinoma cells HepG-2 was used as research materials.Methods This research observed morphological alterations using phase contrast microscopy and electron microscopy, cell proliferation were detected by MTT assay, and using flow cytometry detected apoptotic rates, cell cycle progression, mitochondrial trans-membrane potential and intracellular calcium homeostasis.ResuIts Apoptotic cells appeared morphological alterations.Phloretin exerted a inhibitory the proliferation of HepG-2 cell line, and induced its apoptosis in a dosage and duration dependent manner.Cell cycle was arrested at G1 phase, mitochondrial trans-membrane potential dropped, intracellular free Ca2 + increased.ConcIusion Phloretin can induce apoptosis of HepG-2 via arresting cell cycle progression, reducing mitochondrial trans-membrane potential and disturbing intracellular calcium homeostasis.
