Effect of Genistein combined with cyclosporine A on CXCR3 in rejection of cardiac allograft
10.3969/j.issn.1000-484X.2015.07.006
- VernacularTitle:Genistein联合环孢素 A 对大鼠心脏移植排斥反应中 CXCR3表达的干预
- Author:
Jian ZHANG
;
Guangmin SONG
;
Xiao BAI
;
Lianlong HU
;
Tinglei ZHAO
;
Xin ZHAO
- Publication Type:Journal Article
- Keywords:
Graft rejection;
Genistein;
Cyclosporine A;
CXCR3;
Rats
- From:
Chinese Journal of Immunology
2015;(7):895-897,905
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect of Genistein combined with cyclosporine A on chemokine receptor CXCR3 in rejection of cardiac allograft in rats.Methods:Heterotopic cervical heart transplantation was performed from Wistar rats to SD rats by using cuff-technique.The SD rat recipients were randomly divided into 3 groups.No treatment was adopted in the acute rejection( AR) group.Rats in the cyclosporine A(CsA) group were treated with cyclosporine A after transplantion.Rats in CsA+Genistein(C+G) group were treated with Genistein combined with cyclosporine A after transplantion.All the cardiac allografts were harvested at 7th day, and made into tissue slices.The HE-staining was used to observe the pathology changes of the allograft myocardia.And the expression of CXCR3 was detected with immunehistochemistry and Western blot.Results:The expression of CXCR3 strongly positively expressed in AR group.The degree of myocardial inflammation in C+G group and the expression of CXCR3 were much lower than the other two groups.Conclusion:Genistein combined with cyclosporine A can significantly relieve rejection of cardiac allograft by inhibiting the ex-pression of CXCR3.
