Role of Bcl-2 signal pathway in apigenin preconditioning against cardiomyocytes anoxia/reoxygenation injury
10.3969/j.issn.1001-1978.2015.01.026
- VernacularTitle:Bcl-2介导的芹菜素抗心肌细胞缺氧/复氧损伤作用
- Author:
Min ZHOU
;
Jiazhen YOU
;
Huan HE
;
Dan LIU
;
Zhangping LIAO
;
Lei TANG
;
Dong YIN
;
Ming HE
- Publication Type:Journal Article
- Keywords:
apigenin;
Bcl-2;
anoxia/reoxygenation injury;
cardiomyocytes protection;
apoptosiss;
mito-chondrial
- From:
Chinese Pharmacological Bulletin
2015;(1):122-126,127
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the relationship be-tween the cardioprotection of apigenin ( Api ) from an-oxia/reoxygenation ( A/R) injury and Bcl-2 pathway. Methods H9 c2 cardiomyocytes were cultured and di-vided into normal control group, A/R group, Api pre-treatment group ( Api ) , Api + Bcl-2 inhibitor group ( Api + ABT-737 ) . Expression of Bcl-2 was deter-mined by Western blot,and cell viability was measured by MTT method. LDH, SOD, GSH-Px, MDA activity were determined by chromometry. ROS generation, mi-tochondrial membrane potential and apoptosis were de-termined by flow cytometry. Results 25h after apige-nin precondition,the expression of Bcl-2 was upregulat-ed in cardiomyocytes ( P <0. 01 ) . In the group pre-treated with 40 μmol · L-1 apigenin before A/R, the activity of LDH in culture medium decreased; the ac-tivity of intracellular SOD, GSH-Px increased; the content of MDA and ROS generation decreased; cell viability increased; mitochondrial membrane potential could be more stable and cell apoptosis decreased ( P<0. 01 ) . However, all these protective effects were attenuated significantly in the group pretreated with apigenin and Bcl-2 inhibitor ABT-737 . Conclusion The effect of apigenin against A/R injury in cardiomyo-cytes involves Bcl-2 pathway, and at least partly de-pends on its effect on upregulating the expression of Bcl-2 .
