Determination of Two Andrographolide Derivatives in Andrographolide Sulfonates by UPLC
- VernacularTitle:UPLC法同时测定穿心莲内酯磺化物中2种内酯衍生物的含量
- Author:
Yanhong LIU
;
Fan CHENG
;
Difa LIU
;
Xiaoling YANG
- Publication Type:Journal Article
- Keywords:
UPLC;
Andrographolide sulfonates;
Andrographolide sulfonate E;
Andrographolide sulfonate F;
Determination
- From:
China Pharmacist
2014;(11):1833-1834,1835
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish an UPLC method for the simultaneous determination of andrographolide sulfonate E and an-drographolide sulfonate F in andrographolide sulfonates. Methods: The sample was separated on a Shimadzu Shim-pack XR-ODSⅢcolumn (2. 0 m × 75 mm, 1. 6 μm) with acetonitrile( A) and potassium dihydrogen phosphate buffer solution( B:1. 361 g potassium dihydrogen phosphate and 1. 0g heptane-1-sodium sulfonate were dissolved in 1 000 ml water) as the mobile phase with gradient elu-tion, and the flow rate was 0. 4 ml·min-1 ,the column temperature was 30℃, and the detection wavelength was set at 225nm. Re-sults:The linear range of andrographolide sulfonate E was 4. 39-43. 88μg·ml-1(r=1. 000 0) and the average recovery was 98. 71%(RSD=1. 41%, n=6). The linear range of andrographolide sulfonate F was 4. 43-44. 32 g·ml-1(r=1. 000 0) and the average re-covery was 99. 29%(RSD=0. 76%, n=6). Conclusion:The method is reproducible, sensitive and accurate, which can be used in the quality control of andrographolide sulfonates.
