Effect of bifunctional RDP-BDNF fusion protein on ability of learning and memory of cognitive dysfunction mice induced by scopolamine
10.3969/j.issn.1001-1978.2014.11.018
- VernacularTitle:双功能融合蛋白RDP-BDNF对东莨菪碱所致认知功能障碍小鼠学习记忆的影响
- Author:
Miaomiao ZHANG
;
Enqi ZHANG
;
Feiyan GAO
;
Jing WU
;
Songtao XIANG
;
Ailing FU
- Publication Type:Journal Article
- Keywords:
RDP-BDNF;
blood-brain barrier;
water maze test;
cognitive dysfunction;
cholinergic nerve;
cell-penetrating peptide
- From:
Chinese Pharmacological Bulletin
2014;(11):1569-1573,1574
- CountryChina
- Language:Chinese
-
Abstract:
Aim Through identification of the difunc-tionality of neuroprotection and passage of blood-brain barrier ( BBB) for RDP-BDNF fusion protein to offer a new strategy to treat brain diseases. Methods BDNF was conjugated to a novel cell penetrating peptide known as RDP which was derived from rabies virus gly-coprotein ( RVG) . The fusion protein RDP-BDNF was expressed in E. coli BL21 ( DE3 ) . After the intrave-nous injection of RDP-BDNF, the time effect curve of RDP-BDNF in the brains and serum was examined. Additionally , Morris water maze test was used to evalu-ate the effect of RDP-BDNF on scopolamine-induced amnesic mice, and the mechanism was also studied. Results RDP-BDNF could cross the BBB and exhibi-ted neuroprotective effects on the treatment of cognitive deficit induced by scopolamine in dementia model mice. Conclusions Delivery of protein therapeutics using RDP might offer a new and exciting strategy to treat brain diseases.
