Determination of Berberine Hydrochloride in Shenshe Ointments by HPLC
- VernacularTitle:HPLC法测定参蛇软膏中盐酸小檗碱的含量
- Author:
Shan MIAO
;
Yan LI
;
Jie LI
;
Linlin BI
;
Ya ZHANG
;
Xiaopeng SHI
- Publication Type:Journal Article
- Keywords:
Shenshe ointments;
HPLC;
Berberine hydrochloride;
Determination
- From:
China Pharmacist
2014;(9):1576-1577,1578
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish the determination method for berberine hydrochloride in Shenshe ointments. Methods: The quantitative analysis of berberine hydrochloride in Shenshe osintments was carried out by HPLC with a Kromsal C18 chromatographic column (250 mm × 4. 6 mm, 5 μm), the mobile phase was acetonitrile-0. 1% phosphoric acid (49∶51) (adding 0. 2g sodium dode-cylsulphate into 100 ml solution) with the flow rate of 1. 0 ml·min-1 and the detection wavelength of 320nm, the temperature of col-umn was room temperature, and the injection volume was 20 μl. Results: The linear range was 0. 059 2-0. 296 0 g·L-1 with good correlation(r=0. 999 4). The average recovery was 99. 80% and RSD was 0. 24%(n=6). Conclusion: The established method is accurate, specific and reproducible, and suitable for the determination of berberine hydrochloride in Shenshe ointments.
