Influence of inhibiting p38MAPK expression on TNF-α expression and myocardial cell apoptosis in rats with ischemia/reperfusion injury
10.3969/j.issn.1008-0074.2013.06
- VernacularTitle:抑制p38MAPK表达对大鼠缺血再灌注损伤心肌中TNF-α表达及细胞凋亡的影响
- Author:
Yongguo LIU
;
Peng REN
;
Guoqing LI
- Publication Type:Journal Article
- Keywords:
Myocardium;
Reperfusion injury;
p38 mitogen-activated protein kinases
- From:
Chinese Journal of cardiovascular Rehabilitation Medicine
2013;22(6):538-542
- CountryChina
- Language:English
-
Abstract:
Objective: To explore influence of p38 mitogen-activated protein kinase (p38MAPK) inhibitor on myocardial cell apoptosis and expression of tumor necrosis factor (TNF)-α in rats with ischemia/reperfusion (I/R) injury. Methods: According to number table method, a total of 30 SD rats were randomly and equally divided into blank control group, I/R group and inhibitor group. p38MAPK mRNA expression, TNF-α level and myocardial cell apoptotic rate were measured, compared and analyzed among three groups. Results: Compared with blank control group, there were significant increase in TNF-α level [(3.68±0.16) μg/L vs. (5.02±0.09) μg/L], p38MAPK mRNA expression [(1.76±0.46) vs. (2.35±0.02)] and myocardial cell apoptotic rate [-(3.51±0.40) % vs. - (1.8±0.23) %] in I/R group, P=0.001 all. Compared with I/R group, there were significant decrease in p38MAPK mRNA expression (2.09±0.16), TNF-α level [(4.15±0.11) μg/L] and myocardial cell apoptotic rate [-(2.9±0.50) %] in inhibitor group, P=0.001 all. Conclusion: Inhibition of p38 mitogen-activated protein kinase expression in myocardium of rats can decrease production of tumor necrosis factor-α and myocardial cell apoptosis, then relieve ischemia/ reperfusion injury of myocardial cells.
- Full text:P020140110383926590139.doc
