Curcumin Antagonizes Rotenone-induced Injury of PC12 Cells by Antioxidant Activity
10.3870/j.issn.1672-0741.2010.01.010
- VernacularTitle:姜黄素通过抗氧化作用拮抗鱼藤酮致PC12细胞损伤的研究
- Author:
Qunli CUI
;
Shenggang SUN
- Publication Type:Journal Article
- Keywords:
curcumin;
rotenone;
PC12 cells;
superoxide dismutase;
reactive oxygen species
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2010;39(1):37-41,46
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the cytoprotection of curcumin against rotenone(Ro)-induced injury and the molecular mechanisms underlying in PC12 cells.Methods The insulted model of PC12 cells was established with Ro.Cell viability was determined using MTT reduction assay.The content of reactive oxygen species(ROS)was determined by the method of DCFH-DA staining.Chromatometry was used to measure the total activity of SOD,DCFH-DA staining to measure the level of intracellular ROS,and flow cytometry to assay the apoptosis rate.Results 0.5 and 1.0 μmol/L curcumin significantly decreased the inhibitory rate of Ro on the growth of PC12 cells for 24 h as compared with the Ro group(P＜0.01).0.5 and 1.0 μmol/L curcumin significantly ameliorated the changes in morphology of PC12 cells,increased the activities of intracellular SOD as compared with the Ro group(P＜0.05),decreased the production of intracellular ROS and inhibited the apoptosis of PC12 cells induced by Ro for 24 h as compared with Ro group(P＜0.01).Conclusion Curcumin can resist Ro-induced cytotoxicity probably by the mechanism of scavenging intracellular ROS and increasing the activity of antioxidase.
