The Effect of Histone Deacetylases-1 siRNA on the Growth and Apoptosis of HeLa Cells
10.3870/j.issn.1672-0741.2010.01.012
- VernacularTitle:组蛋白去乙酰化酶1 siRNA对HeLa细胞生长和凋亡的影响
- Author:
Na LI
;
Shiying YU
- Publication Type:Journal Article
- Keywords:
histone deacetylases-1;
siRNA;
cervical cancer;
apoptosis
- From:
Acta Medicinae Universitatis Scientiae et Technologiae Huazhong
2010;39(1):47-49,58
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of histone deacetylases-1(HDAC-1)siRNA on the HDAC-1 protein expression,cell growth and apoptosis in HeLa cells.Methods The HDAC-1 protein was knocked down by HDAC-1 siRNA.HDAC-1 protein was detected by Western blot.The cell growth inhibition was assesses by MTT and clone forming assay.Apoptosis was measured by flow cytometry.Results Forty-eight h after transfection of HDAC-1 siRNA,HDAC-1 protein expression in HeLa cells was down-regulated obviously.The down-regulation of HDAC-1 significantly reduced the colony formation rate,inhibited cell proliferation and induced apoptosis in HeLa cells.Conclusion HDAC-1 siRNA may play an anti-proliferation role in HeLa cells by inducing apoptosis.
