Capsaicin Inhibits Angiotensin Ⅱ Induced Vasoconstriction in Mice
- VernacularTitle:辣椒素抑制血管紧张素Ⅱ介导的血管收缩
- Author:
Dachun YANG
;
Shuangtao MA
;
Liqun MA
;
Zhidan LUO
;
Xiaoli FENG
;
Lijuan WANG
;
Tingbing CAO
;
Zhencheng YAN
;
Daoyan LIU
;
Zhiming ZHU
- Publication Type:Journal Article
- Keywords:
Mouse;
Transient receptor potential vanilloid 1;
Angiotensin Ⅱ;
Vasoconstriction
- From:
Chinese Journal of Hypertension
2007;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the long-term effect of administration(6 months) with transient receptor potential vanilloid 1(TRPV1) agonist capsaicin on contractile reactivity of thoracic aorta in C57BL/6J mice.Methods Tow-month-old male C57BL/6J mice were received normal diet group(n=12) or capsaicin group(normal diet plus capsaicin,n=12).Tail-cuff systolic blood pressure(SBP) was examined at the baseline and at the end of the intervention.After 6-month treatment period,carotid artery blood pressure and heart rate were determined by catheterization,and the aortic contractile response was examined using isometric myograph(Danish Myotech Technology,Denmark).Plasma levels of renin,angiotensin Ⅱ(Ang Ⅱ) and aldosterone were determined.Vascular smooth muscle cells(VSMC) were obtained from thoracic aorta of mice and cultured.Angiotensin Ⅱ type 1 receptor(AT1R) protein expression was detected by western blot.Calcium imaging was detected in cultured VSMC using the fluorescent dye technique.Results Systolic blood pressure,invasive carotid artery blood pressure and heart rate have no difference between two groups.No differences was found in PE-induced contraction response in thoracic aorta;while Ang Ⅱ induced contractility of aortic ring was lower in mice with capsaicin than control group [capsaicin:(37.5?1.6)% vs(59.8?1.4)%,P
