Effect of berberine treatment on the expressions of oxLDL,CD36 and PPAR? of macrophages from diabetic rats
- VernacularTitle:小檗碱对2型糖尿病大鼠巨噬细胞ox-LDL、CD36和PPAR?的影响
- Author:
Sanmei CHEN
;
Min XU
;
Fang WANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus;
Berberine;
Macrophage;
Low-density lipoprotein,oxidative;
Antigen,CD36;
Peroxisomal proliferator-activated receptor
- From:Chinese Journal of Diabetes
2008;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of berberine on the lipid metabolism and the expression of ox-LDL,CD36 and PPAR? in the peritoneal macrophage(PM) and alveolar macrophage(AM) from the type 2 diabetic rats. Methods Sprague-Dauley rats were randomly divided into groups of normal control,high-fat diet,type 2 diabetes and berberine treatment.The levels of blood glucose,insulin and lipid were measured biochemically,the content of ox-LDL in macrophage and the protein expression of CD36 and PPAR? in macrophage were assayed by ELISA,the mRNA expression of CD36 and PPAR? in macrophage was disclosed by RT-PCR. Results Compared with the control group, the levels of glucose,insulin and total cholesterol (TC),triglycerides(TG),low density lipoprotein-cholesterol (LDL-C) in blood were increased significantly(P0.05). The content of ox-LDL,the protein and mRNA expressions of CD36 and PPAR? in PM and AM increased in type 2 diabetic group(P
