Protective effect of mycophenolate mofetil to renal tissures in diabetic rats
- VernacularTitle:霉酚酸酯对糖尿病大鼠肾脏的保护作用
- Author:
Xiaoli HUANG
;
Yongman LV
;
Jianfeng LUO
;
Yan YI
- Publication Type:Journal Article
- Keywords:
mycophenolate mofetil;
diabetic nephropathy;
intercellular adhesion molecule-1;
proliferating cell nuclear antigen
- From:
Basic & Clinical Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To assess the renal protective effects of mycophenolate mofetil(MMF) in diabetic model rats and to explore its mechanism. Methods SD rats were divided randomly into there groups: control group,diabetic model group and diabetic group treated with MMF. In 12 weeks, blood glucose(BG), blood urea nitrogen(BUN), serum creatinine(Scr), index number of kidney hypertrophy(kidey weight to body weight, KW/BW),creatinine clearance(Ccr) and 24- hour urinary protein (24Upro) were detected. Kidney tissure were examined by microscopy. The protein exprssion of intercellular adhesion molecule-1 (ICAM-1) and proliferating cell nuclear antigen(PCNA) in renal tissure were determined by immunohistochemical technique. The mRNA expression of ICAM-1 in kidney tissure were semi-quantitatively determined with reverse transcription-polymerase chain reaction(RT-PCR). Results Compared with control group, BG、KW/BW、24Upro、BUN、Scr and Ccr were significantly increased in diabetic model rats(P
