Studies on the Effects of Ultrasound on Pepsin,Trypsin and Catalase
- VernacularTitle:超声对胃蛋白酶,胰蛋白酶,过氧化氢酶作用的研究
- Author:
Kang WANG
- Publication Type:Journal Article
- Keywords:
Ultrasound Enzyme activity Pepsin Trypsin Catalase
- From:
China Biotechnology
2006;0(05):-
- CountryChina
- Language:Chinese
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Abstract:
Three kinds of enzymes,pepsin,trypsin and catalase,were selected to research the effects and mechanisms of ultrasound on protein,taking advantage of the change of their biological activity under sonication as the index.The results indicate that the three kinds of enzymes display different modes and degrees of activity change under sonication.In addition,the effects of ultrasound on the enzymes vary with their concentration,which means that the destruction of ultrasound to enzyme could be reduced by adjusting their concentration.Mannitol,a strong free radical scavenger,and Tween-80,a kind of non-ionic surfactant,could protect the activity of the enzymes during sonication.That implies that free radicals,which are produced through the thermal dissociation of water induced by ultrasound cavitation,and the cavitation bubbles,which could disturb the hydrophobic environment around the enzyme molecules,play important roles during the destruction of sonication to enzymes.However,for different enzyme,different factor play the main role.
