Culture,identification and differentiation into osteoblast and adipocyte cells from umbilical cord blood-derived mesenchymal-like stem cells
- VernacularTitle:脐血间充质干细胞的分离鉴定及其向脂肪和成骨的分化
- Author:
Zuohua CHI
;
Yuan ZHANG
;
Yan LU
- Publication Type:Journal Article
- Keywords:
umbilical cord blood;
mesenchymal stem cells;
differentiation
- From:
Basic & Clinical Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objects To observe whether mesenchymal stem cells exist in umbilical cord blood and if they can differentiate into osteoblasts and adipocytes.Methods Nucleated cells were separated with Hespan,Mesenchymal-like stem cells were identified by surface marker with flow cytometry.Osteoblast was identified by von Kossa staining and alkaline phosphatase staining,adipocyte was identified by Oil Red O staining.Results From the Nucleated fraction of UCB,we demonstrated the presence of a subset of cells that express adhesion molecules CD13+,CD29+,and CD44+,but not antigens of hematopoietic CD34,CD45,CD14 and neither antigens of endothelia CD106.Exposure of these cells to osteogenic inductive agents resulted in an increase in the expression of alkaline phosphatase and the appearance of hydroxyapatite nodules by Von Kossa staining.Incubation with adipogenic inductive agents resulted in morphological change and positive staining with oil Red O.Conclusions Mesenchymal-like stem cells exist in cord blood and have a lower precursor frequency.The cells can differentiate into osteoblast and adipocyte.Cord blood may function as a new source of cells for cellular therapeutics.
