Transient ischemia and reperfusion induce the expression of glucose transporters GLUT1 and GLUT4 in rat myocardium
- VernacularTitle:短暂缺血再灌注促进心肌葡萄糖转运体基因表达
- Author:
Jing WANG
;
Tao HANG
;
Shisen JIANG
- Publication Type:Journal Article
- Keywords:
Glucose transporters;
Myocardial ischemia;
Reperfusion injury
- From:
Journal of Medical Postgraduates
2005;0(S1):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective : To investigate the dynamic changes of the expression of GLUT1 mRNA and GLUT4 mRNA in rats myocardium with transient ischemia and reperfusion, and the relationship between the dynamic changes and the time during reperfusion. Methods : In rats, the left anterior descending coronary artery was occluded for 20 min followed by reperfusion for 4 hours, 1, 3 or 7 days as ischemia reperfusion model. The relative content of GLUT1 mRNA and GLUT4 mRNA in myocardium was detected by RT-PCR and gel electrophoresis imaging. Results: During myocardial post-ischemia and reperfusion, the levels of GLUT1 mRNA got up to the peak at 4th hour[ (0.666?0. 003 ) vs (0. 509?0.002) controls , P 0.05). Conclusion; Transient ischemia and reperfusion induce the expression of glucose transporters GLUT1 and GLUT4 genes in rat myocardi- um, which contribute to promote glucose utilization during ischemia, protect ischemic myocardium and improve functional recovery on reperfusion.
