Apigenin-induced apoptosis of bladder cancer cell BIU-87 and its mechanism
- VernacularTitle:芹菜素诱导膀胱癌细胞株BIU-87凋亡及机制的研究
- Author:
Shanhua YAO
- Publication Type:Journal Article
- Keywords:
Apigenin;
Bladder cancer;
Apoptosis
- From:
Journal of Chongqing Medical University
2007;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the apoptosis of bladder cancer cell BIU-87 induced by apigenin in vitro and its mechanism. Methods:The cell proliferation was detected by MTT assay;the cell cycle was measured by flow cytometry;the cell apoptosis was observed by flow cytometry with Annexin V-FITC/PI double staining;the expression of survivin mRNA was detected by RT-PCR,and the expressions of survivin protein and caspase-3 protein were detected by immunocytochemistry SP assay.Results:MTT showed that apigenin effectively inhibited the proliferation of BIU-87 cells.②After 72 hours of 22.03?mol/L apigegin treatment,it could arrest the BIU-87 cell in G2/M phase which was measured by flow cytometry,and it could make the cell apoptosis which was showed by flow cytometry with Annexin V-FITC and PI.③Compared with the control group,the expression of survivin mRNA was significantly decreased (P
