Anti-inflammatory Effect of Apigenin on Microglia after Oxygen Glucose Deprivation and Reperfusion
- VernacularTitle:芹菜素对氧糖剥夺损伤小胶质细胞的抗炎作用
- Author:
Zeyi LI
;
Shuzhen HAN
;
Guo WANG
- Publication Type:Journal Article
- Keywords:
oxygen glucose deprivation;
apigenin;
microglia;
IL-1?;
TNF-?
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the neuroprotective effect of apigenin on microglia which was exposed to oxygen glucose deprivation and reperfusion (OGD/R), which was characterized by its influence on IL-1? and TNF-? expression. Methods Primary microglial cultures were prepared from newborn rat brain. The purity of isolated cells were identified by GSA-IB4. The cells were randomized into 5 groups:normal group, DMSO group and apigenin-treated groups (10, 25, 50 ?mol/L). The cells of DMSO group and apigenin-treated group were exposed to 8 h of OGD and 24 h of reperfusion in the presence or absence of apigenin at a range of concentrations. Culture supernatants were collected and IL-1? and TNF-? were detected by ELISA assay. Results The expression of IL-1? and TNF-? were significantly higher in DMSO group (P
