Determination of Chlorogenic Acid in Xiao'er Yinniu Granula by HPLC
- VernacularTitle:HPLC法测定小儿银牛颗粒中绿原酸的含量
- Author:
Zuoping ZHANG
;
Wei HUANG
;
Jianwei YIN
;
Haiyong REN
;
Rong SUN
- Publication Type:Journal Article
- Keywords:
Chlorogenic acid;
Xiao'er Yinniu Granula;
Content determination;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
2000;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop an HPLC method for the determination of chlorogenic acid in Xiao'er Yinniu Granula.Methods A Phenomenex ODS C18 column (4.6 mm?250 mm,5 ?m) was used,column temperature was at 20 ℃,methanol-0.4 %phosphoric acid water solution(20:80)was used as the mobile phase,the flow rate was 1 mL?min-1,and the detection wavelength was 327 nm.Results The calibration curve of chlorogenic acid was linear between 0.284～1.704 ?g (r=0.999 6),the average recovery and the relative standard deviation were 96.19 %and 1.16 %(n=5),respectively.Conclusion The method is simple,accurate and reproducible,by whihc chlorogenic acid can be better separated from Xiao'er Yinniu Granula.This method can be used for quality control of Xiao'er Yinniu Granula.
