Determination of three kinds of berberine type alkaloids in Rhizoma Coptidis and Jinqi Jiangtang Tablet by RP-HPLC
- VernacularTitle:RP-HPLC同时测定黄连及金芪降糖片中3种小檗型生物碱
- Author:
Na NING
;
Jing ZHOU
;
Fang LIU
;
Zhuoyan LI
;
Mo YANG
;
Xin WANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Rhizoma Coptidis;
Jinqi Jiangtang Tablet;
berberin type alkaloids
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish a reversed-phase HPLC method of determining three kinds of berberine type alkaloids(jatrorrhizine,palmatine and berberine) in Rhizoma Coptidis and Jinqi Jiangtang Tablet(Flos Lonicerae japonicae,Rhizoma Coptidis and Radix Astragali) METHODS: RP-HPLC was used for determination.The separation was carried out on Diamonsil C_(18)column(4.6 mm?250 mm,5 ?m) with the mobile phase containing acetonitrile-0.1 mol/L potassium dihydrogen phosphate(30(∶)70) and a detective wavelength was at 345 nm.The flow rate was 1.0 mL/min. RESULTS: The average contents of jatrorrhizine,palmatine and berberine in Rhizoma Coptidis and Jinqi Jiangtang Tablet were 1.34%,1.61%,7.12% and 0.35%,0.64%,1.54% respectively. CONCLUSION: The method is simple,accurate with a good recovery and repeatability and can be used to control the quality of Rhizoma Coptidis and Jinqi Jiangtang Tablet.
