Content Determinate of Berberine Hydrochloride in Sifangwei Capsules by HPLC
- VernacularTitle:HPLC法测定四方胃胶囊中盐酸小檗碱的含量
- Author:
Dongping GUO
;
Qibin LIANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Sifangwei capsules;
berberine hydrochloride;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the content determination method of berberine hydrochloride in Sifangwei capsules.Methods HPLC method was used with octadecylsilane chemically bonded silica as the filler,acetonitrile-0.05 mol/L KH2PO4 solution(25∶75) as the mobile phase,detective wavelength was 230 nm.The number of theoretical plates should not be less than 3 000.Results The content determination method has a good linear relation at the range of 0.201 6～1.008 ?g(r=0.999 8).The recovery rate was 98.7%,RSD=1.24%(n=5).Conclusion The method is simple,accurate,with good separation and reproducibility,and can be used for the quality control of Sifangwei capsules.
