Blade differentiation and plantlet regeneration of Gynostemma pentaphyllum
- VernacularTitle:绞股蓝叶片分化与植株再生
- Author:
Ruiqiang ZHAO
;
Zhengzhu LING
;
Yu LUO
;
Xiongying LI
;
Junfu JIANG
;
Yaosheng WU
- Publication Type:Journal Article
- Keywords:
Gynostemma pentaphyllum(Thunb.) Makino;
blade differentiation;
plantlet regeneration
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To construct a stably-performing and high-efficiency regeneration system of Gynostemma pentaphyllum and lay a foundation for the gene transformation of it.Methods The blades of five-leaf G.pentaphyllum were used as the explants and cultured in MS media with different portions of hormone to induce fascicled-bud,root and plantlet regeneration.Results The medium suitable for inducing the blade differentiation of G.pentaphyllum was MS+6-BA 1.0 mg/L and for the frequency of blade differentiation could reach 40%.The cultural medium MS+6-BA 1.0 mg/L was suitable for the sub-multiplication of fascicled-bud and the medium 1/2 MS for root inducement and the plantlet regeneration.Conclusion A stably-performing acceptor system of the direct differentiation for Agrobacterium tumefaciens mediated gene transformation of G.pentaphyllum blades is constructed.
