Content Determination of Chlorogenic Acid in Shuanghua Eardrop by HPLC
- VernacularTitle:HPLC法测定双花滴耳液中绿原酸的含量
- Author:
Futian WANG
;
Xi YANG
;
Luqi HUANG
- Publication Type:Journal Article
- Keywords:
Shuanghua eardrop;
chlorogenic acid;
HPLC
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method of content determination of chlorogenic acid in Shuanghua eardrop. Methods High performance liquid chromatography (HPLC) was adopted with Kromasil C18 column (5 ?m,4.6 mm?250 mm) and acetonitrile-0.4% phosph acid solution (10∶85) as the mobile phase. The detective wavelength was 328 nm,the flow rate was 1.0 mL/min,column temperature was room temperature. Results Average recovery was 99.8%,relative standard error was 1.51% (n =5). Conclusion The linearity relationship of chlorogenic acid was good at the range of 0.102 4～0.061 44 ?g.
