Determination of chlorogenic acid and caffeic acid in Fanhuncao Granule by RP-HPLC
- VernacularTitle:RP-HPLC测定返魂草颗粒中绿原酸和咖啡酸含量
- Author:
Yanqiang LIU
;
Hongwu WANG
;
Xiange LU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Fanhuncao Granule;
chlorogenic acid;
caffeic acid
- From:
Chinese Traditional Patent Medicine
1992;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develope a reversed-phase high performance liquid chromatographic method for determing chlorogenic acid and caffeic acid in Fanhuncao Granule(Senecio cannabifolius Less). METHODS: The operation was carried out on Eclipse XDB-C_(18) column with the mobile phase consisting of a mixture of acetonitrile-0.4% phosphoric acid(12(∶)88,V/V).The flow rate of 1.0 mL/min and UV detection wavelength at 327 nm were set to determine the contents of chlorogenic acid and caffieic acid. RESULTS: There was good linear relationship between the concentrations and the peak-areas of chlorogenic acid and caffeic acid.The two kinds of standard solutions were both stable in 16 h(RSD=1.55% for chlorogenic acid,1.11% for caffeic acid) The average recovery was 100.1% and 99.8% for chlorogenic acid and caffeic acid,repectively. CONCLUSION: The method is simple,sensitive,rapid and accurate,and can be used for the quality control of Fanhuncao Granule.
