RP-HPLC Determination of Palmatine Hydrochloride and Berberine Hydrochloride in the Preparation of Fu Yan Xiao Lotion
- VernacularTitle:HPLC法测定妇炎消洗剂中盐酸小檗碱和盐酸巴马汀的含量
- Author:
Xiaofeng XU
;
Yongheng TAN
;
Jianhong LIU
- Publication Type:Journal Article
- Keywords:
Fu Yan Xiao Lotion;
Palmatine hydrochloride;
Berberine hydrochloride;
HPLC.
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the concents of palmatine hydrochloride and berberine hydrochloride in the preparation of Fu Yan Xiao Lotion(FYXL).Methods The HPLC method was established for the determination of palmatine hydrochloride and berberine hydrochloride in the preparation of FYXL.The chromatographic column is Agilent Extend C18(4.6mm? 150 mm,5 ? m),mobile phase consists of water-acetonitrile(40 ∶ 60,3.5 g potassium dihydrogen phosphate and 1.5 g sodium dodecyl sufate per 1 000 mL),column tempreture was 30 ℃,and the flow rate was 1.0 mL/min.The detection wavelength was 345 nm.Results A good linearity is obtained in the range of 0.062 5 ? g to 1.250 ? g of berberine hydrochloride,and 0.068 6～ 1.372 ? g of palmatine hydrochloride.The average recovery of berberine was 98.63 %,RSD being 2.01 %.The average recovery of palmatine was 102.34 %,RSD being 1.67 %.Conclusion This method is simple and quick,and can be used for the quality control of FYXL.
