HPLC Determination of Chlorogenic Acid Content in Yinqiao Jiedu Oral Liquid
- VernacularTitle:HPLC法测定银翘解毒口服液中绿原酸的含量
- Author:
Jingjin ZHONG
;
Fenglian CHEN
;
Shuling WANG
;
Cheng CAO
;
Xianfang YANG
- Publication Type:Journal Article
- Keywords:
Yinqiao Jiedu Oral Liquid;
Chlorogenic acid;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the HPLC determination method of chlorogenic acid for developing the quality standard of Yinqiao Jiedu Oral Liquid.Methods HPLC method was performed on an E.Merck LiChrospher 100RP-18 column(250 mm? 4 mm,5 ? m).The mobile phase consisted of acetonitrle and o.4 % phosphoric acid(10:100).The flow rate was 1.0 mL/min,column temperature was 30 ℃,and the detection wavelength was 327 nm.Results Chlorogenic acid was linear at the range of 0.12～ 0.63 ? g(r=0.999 6),the average recovery was 96.68 %,and RSD was 1.56 % .Conclusion This method is accurate,reliable,reproducible,and can provide evidence for the quality control of Yinqiao Jiedu Oral Liquid.
