Content Determination of Chlorogenic Acid in David's Blunt by HPLC
- VernacularTitle:高效液相色谱法测定大卫颗粒中绿原酸的含量
- Author:
Xiaoming YANG
;
Lin YANG
;
Jun JIN
- Publication Type:Journal Article
- Keywords:
HPLC;
David's Blunt;
Chlorogenic Acid;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the method of content determination of Chlorogenic Acid in David's Blunt. Methods The content of Chlorogenic Acid in David's Blunt was determined by HPLC. Take Alltima C18 (150 mm?4.6 mm, 5 ?m) as column, mobile phase was water-methyl acetonitrile-acetic acid glacial (95∶ 10∶5∶2); temperature was 35 ℃; wave-length was at 326 nm; flow rate is 1.0 mL/ min. Results Chlorogenic Acid was linear in the range of 10.01～60.06 ?g, r =0.999 9 (n =6). The average recovery rate was 99.03%, RSD=1.12% (n =6). Conclusion The method is simple, credibility, reproducable, and can be used for the quality control of David's Blunt.
