Determination of Capsaicin in Different Kinds of Dried Capsicums by RP-HPLC
- VernacularTitle:HPLC法测定不同品种辣椒药材中辣椒碱的含量
- Author:
Jian LI
;
Xiaoying LONG
;
Juan SUN
;
Junhua ZHANG
;
Huaming ZHANG
- Publication Type:Journal Article
- Keywords:
Capsicum;
Capsaicin;
RP-HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of capsaicin in different kinds of dried capsicums.Methods A RP-HPLC method was established.The chromatographic column was DiamonsilTM C18(250 mm ? 4.6 mm,5 ? m) and the column temperature was 30 ℃.The mobile phase consisted of methnol ∶ 0.1 % phosphoric acid aqueous solution(70 ∶ 30) with a flow rate of 1.0 mL? min-1.The detection wavelength was 281 nm.Results A good linearity of capsaicin was in the rang of 0.100 6～ 4.024 0 ? g.The average recovery was 101.18 % and RSD was 1.81 %(n=6).Conclusion The method can be used to determine the content of capsaicin in different kinds of dried capsicums.
