Determination of contents of berberine type alkaloids in Huanglian Shangqing Pills(Tablets)
- VernacularTitle:黄连上清丸(片)中小檗型生物碱的含量测定
- Author:
Fang LIU
;
Hao ZHANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Huanglian Shangqing Pills(Tablets);
berberine type alkaloids;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To separate and determine berberine type alkaloids in Huanglian Shangqing Pills(tablets)(Rhizoma Coptidis,Fructus Gardeniae,Fructus Forsythiae,Cortex Phellodendri Chinensis,etc.). METHODS: RP-HPLC was used,and the mobile phase consisted of acetonitrile-solution of potassium phosphate(3.4 g?L~(-1),adjust pH to 3.0 with H_3PO_4)(40∶60),with 1.7 g?L~(-1) SDS.The detecting wavelength was at 345 nm. RESULTS: The contents of five kinds of berberine type alkaloids,berberine,palmatine,coptisine,epiberberine and jatrorrhizine in Huanglian Shangqing Pills(tablets) produced by 10 pharmaceutical factories were determined. CONCLUSION: The method is simple,accurate with a good recovery and repeatability.The method can be used to control the quality of Huanglian Shangqing Pills(Tablets).
