A study on porcine acellular cardiac scaffold
- VernacularTitle:脱细胞猪肺动脉带瓣管道的支架材料研究
- Author:
Peng YAN
;
Shengshou HOU
;
Bing REN
- Publication Type:Journal Article
- Keywords:
Pulmonary artery Bioprosthesis Cytapheresis Animal testing alternatives Calcinosis
- From:Chinese Journal of Thoracic and Cardiovascular Surgery
1995;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the change of the structural and biological properties of the cardiovascular tissue after cell extracting. Methods The native and treated pulmonary cusp and pulmonary artery wall were evaluated by HE stain, ETVG stain, immunochemistry stain and scan electron microscopy. The contents of the main macro-molecules were measured. The content and position of calcium was investigated by subcutaneous implantation in a rat model. Results The acellularization procedure resulted in an almost complete removal of the original cells while the construction of the matrix remained. The contents of colleagen and elastin increased while soluble GAG reduced. The content of calcium in acellular tissue by xenogenic implantation was less obviously than that in fresh one. Conclusion A suitable acellularization procedure can remove all cells and most soluble proteins, and matrix remains nearly intact, so as to alleviate obviously calcification via xenogenic implantation in vivo.
