Effect of Glycyrrhiza flavonoids on DNA and RNA in tumor cell of S_(180) and H_(22) tumor-bearing mice
- VernacularTitle:甘草黄酮对S_(180)和H_(22)荷瘤小鼠肿瘤细胞DNA和RNA的影响
- Author:
Yubin JI
;
Wei JIANG
;
Ming SHANG
;
Hongliang WANG
- Publication Type:Journal Article
- Keywords:
Glycyrrhiza flavonoids;
tumor cell;
Laser Scanning Confocal Microscope(LSCM);
(acridine) orange(AO)
- From:
Chinese Traditional and Herbal Drugs
1994;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe effects of Glycyrrhiza flavonoids on DNA and RNA in tumor cell of S_(180) and H_(22) tumor-bearing mice.Methods S_(180) and H_(22) mice were randomly divided into Glycyrrhiza flavonoids(25,11.25,and 5.58 mg/kg) groups,positive control(cytoxan 25 mg/kg) group,and negative control(NS) group,whom were given drugs by sc.DNA and RNA in tumor cells were examined,respectively by Laser Scanning Confocal Microscope and fluorescent probe of acridine orange(AO) technology.Results All different dosage of Glycyrrhiza flavonoids,cytoxan reduced the brightness of fluorescence of DNA and RNA;low and high dosage of Glycyrrhiza flavonoids had no significant effect on the fluorescence pixels of RNA/DNA;Both middle dosage of Glycyrrhiza flavonoids and cytoxan had significant effect on the fluorescence pixels of RNA/DNA(P
