Genistein can induce apoptosis of human primary gastric cancer cells implanted in nude mice
- VernacularTitle:三羟基异黄酮诱导人胃癌原代细胞裸鼠移植瘤凋亡的研究
- Author:
Haibo ZHOU
;
Qin DU
;
Yun YAN
- Publication Type:Journal Article
- Keywords:
Genistein;
Cell line, tumor;
Neoplasm transplantation;
Apoptosis
- From:Chinese Journal of Digestion
2001;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of the apoptosis of human primary gastric cancer cells implanted in nude mice induced by genistein. Methods Human primary gastric cancer cells were implanted into Balb/c nude mice to establish the cancer model, and the growth curve of implanted tumor was measured. Twenty-five nude mice with implanted gastric cancer cells were divided 5 groups. Genistein at different doses (0.5,1.0 and 1.5 mg/kg), normal saline and dimethyl sulfoxide were injected around the neoplasm. After treatment, transmission electron microscope and TUNEL staining were used to detect the apoptosis of implanted tumor cells. Immunohistochemical staining and RT-PCR were used to detect the expression of apoptosis-related genes bcl-2 and bax in implanted tumor. Results Genistein could significantly inhibit carcinoma growth when it was injected near the carcinoma. The apoptosis of tumor cells in implanted tumor induced by genistein was detected by transmission electron microscopy. The apoptosis index of the genistein treatment groups (28.7%?1.1%, 33.4%?1.4% and 37.1%?1.0%) was significantly higher than that of control groups(11.8%?0.4% and 11.7%?0.4%; P
