Determination of genistein and quercetin in Huaijiao Pills by RP-HPLC
- VernacularTitle:RP-HPLC测定槐角丸中槲皮素和染料木素的含量
- Author:
Qingquan BIAN
;
Zhenpin YANG
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Huaijiao Pills;
genistein;
quercetin
- From:
Chinese Traditional Patent Medicine
1992;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM To determine the contents of the two free-and conjugated quercetin and genistein in Huaijiao Pills (Fructus Sohorae,Radix Sanguisorbae,Radix Scutellariae,etc.).by RP-HPLC. METHODS HPLC was employed with a column of Diamonsil ODS-C_ 18 (4.6 mm?150 mm,5.0 ?m) and a mobile phase of MeOH-H_2O-HAc(2∶ 3∶ 0.25),the detection wavelength being at 254 nm and the column temperature being at 30?C . RESULTS Nice linear correlation existed, when quercetin was within 0.204 8-1.229 ?g and genistein within 0.532 5-3.192 ?g.The correlation coefficient of each group was 0.999 1 and 0.999 8 respectively.The average recoveries of quercetin and genistein were 99.0 % and 99.1 % ,respectively.The RSDs were 1.02 % and 0.40 % ( n =5),respectively. CONCLUSION With this method,quercetin and genistein in Huaijiao Pills could be determined simultaneously and applicable to the quality control of Huaijiao Pill.
