Inhibition of Neuropeptide-Y-induced Vascular Smooth Muscle Cell Proliferation with Pueraria Lobata Isoflavones
- VernacularTitle:葛根总黄酮对神经肽Y诱导血管平滑肌细胞增殖的抑制作用
- Author:
Hong YAO
;
Shaohua HUANG
;
Ziren SU
- Publication Type:Journal Article
- Keywords:
PUERARIA LOBATA ISOFLAVONES/pharmacology;
CARDIOVASCULAR DISEASES/TCD therapy;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
[Objective] To observe the effect of Pueraria Lobata isoflavones (PLI) on neuropeptide-Y-induced (NPY) vascular smooth muscle cell (VSMC) proliferation. [Methods] Experiment system for VSMC was set up as 4 groups: control group (with DME culture fluid, free of blood serum; group A), model group (with NPY culture fluid; group B), control + PLI group (with PLI culture fluid; group C) and model + PLI group (with NPY and PLI culture fluid; group E). Quantitative fluoroimmunohistochemistry techniques were applied to examine the mean fluorescent values of proliferating cell nuclear antigen (PCNA), platelet derived growth factor (PDGF) and C-myc expression. [Results] The levels of PCNA, PDGF and C-myc expression in model group were higher than control group (P
