Determination of Chlorogenic Acid in Yinren Granules by HPLC
- VernacularTitle:HPLC法测定银仁颗粒中绿原酸的含量
- Author:
Shanxin LIU
;
Yuanyin JIA
;
Xinjun ZHANG
;
Ruixue LIANG
;
Qing LIU
- Publication Type:Journal Article
- Keywords:
Yinren granules;
Chlorgenic acid;
HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the content determination of c hlorogenic acid in Yinren Granules. Methods The content of chlorogenic acid wa s detected by HPLC. HPLC was performed with Shim- Pack CLC- ODS column(150? 6.0 mm, 5 ? m) at room temperature, 3 % methyl alcohol- methanol( 79 ∶ 2 1) as mobile phase, the detection wavelength at 327 nm, the flow rate being 1. 2 mL/min and sample loop volume being 10 ? L. Results Chlorogenic acid has a good linearity within 0.102～ 1.02? g (r=0.999)and the average recovery was 98. 17 % with RSD being 1.31 % . Conclusion This method is simple, accurate and can be used for the quality control of Yinren Granules.
