Determination of Berberine and Phellodendrine in Cortex Phellodendri by HPLC
- VernacularTitle:HPLC法测定黄柏药材中小檗碱与黄柏碱的含量
- Author:
Chenchen ZHU
;
Chaozhan LIN
;
Jianxia MO
- Publication Type:Journal Article
- Keywords:
Cortex Phellodendri;
Berberine;
Phellodendrine;
Content determination;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of Berberine and Phellodendrine in Cortex Phellodendri(CP).Methods Reverse-phase HPLC method was used for determination of Berberine and Phellodendrine in different batches of CP.The chromatographic conditions were:Merck-lichrospher RP-C18 column(4.6?250 mm,5 ?m),flow rate being 0.8 mL/min,column temperature at 25 ℃,detection wavelength at 284nm for Phellodendrine and 245nm for Berberine.Results The content of Berberine was 4 times and that of Phellodendrine in Chuan CP 2～3 times as much as that of Guan CP.Conclusion The method is proved to be feasible for quality assessment of Cortex Phellodendri.Limited contents of Berberine and Phellodendrine should be laid out for the great difference in Chuan CP and Guan CP.
