Determination of berberine hydrochloride in Huanglianshangqing Tablets by HPLC
- VernacularTitle:HPLC测定黄连上清片中盐酸小檗碱的含量
- Author:
Shaoting YU
;
Hongwen HUANG
- Publication Type:Journal Article
- Keywords:
berberine hydrochloride;
Huanglianshangqing Tablets;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(08):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish HPLC method for the determination of berberine hydrochloride in Huanglianshangqing Tablets(Rhizoma Coptidis, Radix et Rhizoma Rhei, Fructus Forsythiae, etc.). METHODS: The Diamonsil TM C 18(20cm?4.6mm,5?m) was used with 0.033mol?L -1 Potassium dihydrogen phosphate solution-acetonitrile (70∶30) as a mobile phase. The flow rate was 1.0 mL?min -1. The detecting wavelength was 265nm. RESULTS: The linear of berberine hydrochloride was in the range of 0.2376～ 0.5544?g, r= 0.9999. The average recovery was 98.7% and RSD was 0.7%(n=5), respectively. CONCLUSION: The method is simple,rapid and accurate.
