Determination of emodin and berberine hydrochloride in Wangshibaochi Pills by HPLC
- VernacularTitle:HPLC测定王氏保赤丸中大黄素和盐酸小檗碱的含量
- Author:
Yufeng XIA
;
Qin YANG
;
Qiang WANG
- Publication Type:Journal Article
- Keywords:
Wangshibaochi Pills;
emodin;
berberine hydrochloride: HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To established a method for determinating emodin and berberine hydrochloride in Wangshi baochi (Pills Radix et Rhizoma Rhei,Rhizoma Coptidis,etc.) by HPLC. METHODS : The separation was performed on Lichrospher C_ 18 column,using methanol-0.1% H_3PO_4(90∶10) and acetonitrile-0.05mol?L -1 KH_2PO_4 (35∶65) as mobile phase,respectively. The detection wavelength was at 290nm and 345nm,respectively. The flow rate was at 1.0mL?min -1 ,the column temperature was room temperature. RESULTS : The linear range of emodin was in the range of 0.1966～0.9830 ?g,r =0.9997, the average recovery was 98.81% and RSD was 1.41%,respectively. The linear range of berberine hydrochloride was 0.1644～0.8220 ?g,r =0.9996,the average recovery was 98.47% and RSD was 2.08%. CONCLUSION : This method is rapid,sensitive and accurate. It can be used for quality control of Wangshi baochi Pills.
