Determination of quercetin and kaempferol in Stamen Neulumbinis by HPLC
- VernacularTitle:HPLC测定莲须中槲皮素和山萘酚的含量
- Author:
Xiangsheng MEN
;
Ming SHA
;
Aimin CAO
;
Li JIANG
;
Tiehon DENG
- Publication Type:Journal Article
- Keywords:
Stamen Neulumbinis;
querctin;
kaempferol;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To determine quercetin and kaempferol in stamen neulumbinis by HPLC. Methods : Shim-pack CLC-ODS column was used. Mobile phase consisted of methanol-0.025% phosphate. UV detector wavelength was set at 370nm. Results : The chromatographic peaks of quercetin and kaempferol and other components were completely separated. The average recovery was 96.8% and RSD was 1.97%. Conclusion : The experiment provided new markers and a reliable method for the quality control of Stamen Neulumbinis.