Determination of galangin and kaempferol in Rhizoma Alpiniae Officinarum by HPLC
- VernacularTitle:HPLC法测定高良姜中高良姜素和山柰素的含量
- Author:
Zhiyong LI
;
Dongmei SUN
;
Jianjun ZHANG
- Publication Type:Journal Article
- Keywords:
Rhizoma Alpiniae Officinarum;
HPLC;
Galangin;
Kaempferol
- From:China Journal of Traditional Chinese Medicine and Pharmacy
2006;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:HPLC was used for the determination of galangin and kaempferol of Rhizoma Alpiniae Officinarum.Methods:The column was phenomenex Gemini C 18 (5?,250mm?4.6mm);the mobile phases were methanol-0.4% phosphoric acid(60:40);the detection wavelength was 360 nm;the flow rate was at 1.0 ml/min and the column temperature was at 30?C respectively.Results:Galangin showed a good linear relationship in the range of 0.668-6.680?g,r=0.9996,and the average recovery was 102.30%with RSD=1.73%(n=6);Kaempferol also showed a good linear relationship in the range of 15.5-155.0ng,r=0.9995,and the average recovery was 97.31%with RSD 1.84%(n=6).Conclusion:This method was simple,accurate,reliable and sensitive and it can be used as a quality control standards for Rhizoma Alpiniae Officinarum.
